BioX cell公司位于美国新罕布什尔州的West Lebanon，具有超过15年的单克隆抗体和重组蛋白生产及定制经验，可为学术界、制药和诊断行业的研究人员提供多种高品质的、临床前级别的单克隆抗体和重组蛋白。
Can I get a free sample of a product?
We do not offer free or trial sized samples for testing purposes.
How do I ship cells to Bio X Cell for custom antibody purification?
First, please fill out and submit a cell line transfer sheet which can be found here. Then ship the cells frozen and packed with plenty of dry ice via FEDEX overnight delivery. Please do not ship on Thursdays. Make sure to put the “Dry Ice” label (black diamond) on the box.
How do I store my antibodies?
Store your antibodies undiluted at 4°C in the dark. We do not recommend freezing antibodies (even when aliquoted) as this can result in a loss of activity caused by the freezing/thawing process. Additionally, diluting antibodies to working concentrations and storing at 4°C for more than a day should be avoided. Proteins in general are less susceptible to degradation when stored at higher concentrations. Under these storage conditions your antibodies should remain active for up to one year and oftentimes longer (just make sure to keep the antibody sterile).
How do I handle my antibodies?
Always try to maintain the sterility of your antibodies to avoid con***ination. Working with the antibodies in a biological safety cabinet and using sterile pipet tips, tubes, syringes, and buffers will greatly reduce the chances of con***ination. Additionally, try to keep the antibodies cold until just prior to administration.
What is the shelf life of Bio X Cell’s products?
Bio X Cell’s products have a minimum guaranteed shelf life of 6 months from the date of receipt if the antibodies are stored under the conditions outlined on our product data sheets.
We have not determined the exact expiration date of our products however, antibodies are very stable proteins and under optimal storage conditions your antibodies should remain active for up to one year and oftentimes longer.
How are Bio X Cell’s antibodies supplied?
Most of our antibodies are supplied as an aqueous solution in PBS containing 20mM NaH2PO4/KHPO4 and 135mM NaCl. However, we can supply antibodies in other buffers when requested. The pH will vary depending on the pI of the individual antibody. The buffer will not contain preservatives such as azide or protein stabilizers such as BSA.
What are the concentrations of Bio X Cell’s antibodies?
The concentration of our products varies from lot to lot but is typically between 4 and 10 mg/ml. The exact concentration will be marked on the tube itself. If you need to know the exact concentration of a product before ordering, please contact email@example.com.
What is the epitope of the antibody?
We have not epitope mapped our antibodies at Bio X Cell. Searching the published literature would be the best way to determine if this information is available.
How do I dilute my antibody to a working concentration?
We recommend diluting our antibodies in the same buffer and at the same pH that they are supplied in. Our antibodies are supplied in PBS containing 20mM NaH2PO4/KHPO4 and 135mM NaCl. The pH will vary depending on the pI of the antibody and will be specified on the product data sheet and product webpage for each antibody.
When diluting we recommend using cold PBS and maintaining sterility by working in a biological safety cabinet and using sterile pipet tips, tubes, syringes, and buffers.
Diluting antibodies to working concentrations and storing at 4°C for more than a day should be avoided.
Are Bio X Cell’s antibodies suitable for in vivo administration?
All of our products are suitable for in vivo use. They are supplied in PBS without preservatives such as azide or protein stabilizers such as BSA. The endotoxin levels are < 2EU/mg (InVivoMab) which qualifies them for most in vivo studies. To meet the most stringent requirements we also offer antibodies with lower endotoxin levels: <1EU/mg (InVivoPlus).
What is the in vivo half-life of your antibody?
We do not have direct experimental data generated at Bio X Cell concerning the half-life of our antibodies in vivo. Antibody half-life can be highly variable and influenced by a variety of factors including species, isotype, antigen distribution, antigen concentration and many others. We recommend reading through the literature associated with the antibody you’re interested in to find published data in an experimental system similar to yours as a first step in estimating the half-life.
What dose or frequency of administration should I use to treat my mice?
The optimal dose and frequency of administration for any given experiment and antibody can vary greatly based on many details including the experimental system (i.e. mouse strain, disease model, etc.), the duration of the experiment, the target tissue, the antigen concentration, and many more. For this reason, the optimal dose is best determined by reading through the product references associated with the antibody you’re interested in to find published data in an experimental system similar to yours. Using this as a starting point you can then optimize the dose and frequency of administration experimentally.
Each of our products has an up-to-date reference list available on the products webpage.
What is the difference between Bio X Cell’s InVivoMab and InVivoPlus product lines?
Please see the table below.
What should I do about floaties, precipitates, or aggregates?
Environmental conditions such as temperature variations, freezing/thawing, shaking during transport or long term storage might lead to protein aggregation. These aggregates are either settled in the conical tip of the vial or are floating freely in the suspension. These aggregates can be removed by gentle shaking at room temperature; they can also be removed by filtration or centrifugation without significant loss.