​ATF-3 Polyclonal Antibody

ATF-3 Polyclonal Antibody 

Catalog No DM-Ab-01554 

Isotype IgG 

Reactivity Human;Mouse;Rat 

Applications WB;IHC;IF;ELISA 

Gene Name ATF3

Protein Name Cyclic AMP-dependent transcription factor ATF-3 

Immunogen The antiserum was produced against synthesized peptide derived from human ATF3. AA range:131-180 

Specificity ATF-3 Polyclonal Antibody detects endogenous levels of ATF-3 protein. 

Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. 

Source Polyclonal, Rabbit,IgG

Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. 

Dilution WB 1:500-2000;IHC-p 1:100-500;IF/ICC 1:100-500;ELISA 1:5000-20000 

Concentration 1 mg/ml Purity ≥90% 

Storage Stability -20°C/1 year

Synonyms ATF3; Cyclic AMP-dependent transcription factor ATF-3; cAMP-dependent transcription factor ATF-3; Activating transcription factor 3 

Observed Band 21kD 

Cell Pathway Nucleus . 

Tissue Specificity Amygdala,Brain,Cerebellum,Muscle,

Function function:This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.,similarity:Belongs to the bZIP family.,similarity:Belongs to the bZIP family. ATF subfamily.,similarity:Contains 1 bZIP domain.,subunit:Binds DNA as a homodimer or a heterodimer.,

Background activating transcription factor 3(ATF3) Homo sapiens This gene encodes a possible that alternative splicing of this gene may be physiologically important in 

matters needing attention Avoid repeated freezing and thawing!

Usage suggestions This product can be used in immunological reaction related experiments. For Western Blot analysis of 293T-UV cells using ATF-3 Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). Western blot analysis of lysates from RAW264.7 cells, using ATF3 Antibody. The lane on the right is blocked with the synthesized peptide. member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is more information, please consult technical personnel. the regulation of target genes. [provided by RefSeq, Apr 2011],Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).