IL-6 Polyclonal Antibody

IL-6 Polyclonal Antibody

Catalog No  DM-Ab-16022

Isotype         IgG

Reactivity         Human;Rat;Mouse

Applications         WB;IHC;IF;ELISA

Gene Name IL6

Protein Name Interleukin-6

Immunogen The antiserum was produced against synthesized peptide derived from the

Internal region of human IL6. AA range:131-180

Specificity IL-6 Polyclonal Antibody detects endogenous levels of IL-6 protein. Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Source Polyclonal, Rabbit,IgG

Purification The antibody was affinity-purified from rabbit antiserum by

affinity-chromatography using epitope-specific immunogen. 

Dilution Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300

Concentration 1 mg/ml

Purity ≥90%

Storage Stability -20°C/1 year

Synonyms IL6; IFNB2; Interleukin-6; IL-6; B-cell stimulatory factor 2; BSF-2; CTL

differentiation factor; CDF; Hybridoma growth factor; Interferon beta-2; IFN-beta-2

Observed Band 23kD

Cell Pathway Secreted . Tissue Specificity Produced by skeletal muscle. Function disease:At least 1 polymorphism in the IL6 gene renders HIV-infected men

Not yet tested in other applications.

susceptible to Kaposi sarcoma [MIM:148000]. Kaposi sarcoma is a sarcoma of

spindle cells mixed with angiomatous tissue. A relatively rare malignant skin

tumor that results in multifocal purplish coloured papules or plaques that

eventually form nodules. Most commonly seen in patients who suffer from

AIDS.,disease:Genetic variations in IL6 are associated with susceptibility to

systemic juvenile rheumatoid arthritis [MIM:604302]. Systemic juvenile

rheumatoid arthritis is juvenile chronic arthritis associated with severe, debilitating, extraarticular features, and occasionally fatal complications. Despite

medical treatment, many children still experience early joint destruction, necessitating surgical replacement.,function:IL-6 is a cytokine with a wide variety

of biological functions: it plays an essential role in the

Jiao, Z., Ma, Y., Zhang, Q. et al. The adipose-derived

mesenchymal stem cell secretome promotes hepatic

regeneration in miniature pigs after liver

ischaemia-reperfusion combined with partial

resection. Stem Cell Res Ther 12, 218 (2021)

Liu, Bowen, et al. "Oncoprotein HBXIP enhances

HOXB13 acetylation and co-activates HOXB13 to

confer tamoxifen resistance in breast cancer." Journal

of hematology & oncology 11.1 (2018): 26.

Liu, Yanmei, et al. "Cancer Stem Cells are Regulated

by STAT3 Signalling in Wilms Tumour." Journal of

Cancer 9.8 (2018): 1486.

Immunofluorescence analysis of Hela cell. 1,Stat3

Polyclonal Antibody(red) was diluted at 1:200(4° overnight). β-tubulin Monoclonal Antibody(M7)(green)

Liu, Yanmei, et al. "Cancer Stem Cells are Regulated

by STAT3 Signalling in Wilms Tumour." Journal of

Cancer 9.8 (2018): 1486.

was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit

Alexa Fluor 594 Catalog:RS3611 was diluted at

1:1000(room temperature, 50min). Goat Anti Mouse

Alexa Fluor 488 Catalog:RS3208 was diluted at

1:1000(room temperature, 50min).