Actin β Polyclonal Antibody

Actin β Polyclonal Antibody 

Catalog No DM-Ab-03079

Isotype IgG 

Reactivity Human;Mouse;Rat;Chicken;Globefish ;Bovine;Hamster;Pig;Ovine;Cat;Pig;Dog;Sheep 

Applications IF;WB;IHC;ELISA 

Gene Name ACTB

Protein Name Actin cytoplasmic 1

Immunogen Synthesized peptide derived from the N-terminal region of human Actin β. AA range: 1-80 

Specificity Actin β Polyclonal Antibody detects endogenous levels of Actin β protein. 

Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. 

Source Polyclonal, Rabbit,IgG

Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Dilution IF: 1:50-200 Western Blot: 1/2000 - 1/10000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. 

Concentration 1 mg/ml 

Purity ≥90% 

Storage Stability -20°C/1 year 

Synonyms ACTB; Actin; cytoplasmic 1; Beta-actin; Actin β;β-Actin;Beta actin

Observed Band 42kD 

Cell Pathway Cytoplasm, cytoskeleton . Nucleus . Localized in cytoplasmic mRNP granules containing untranslated mRNAs. .

Tissue Specificity B-cell lymphoma,Brain,Cajal-Retzius cell,Eye,Fetal brain cortex,Foreskin,Hepatocellular carwww.dumabio.com 

 Function disease:Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) 

Background This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a 

matters needing attention Avoid repeated freezing and thawing! 

Usage suggestions This product can be used in immunological reaction related experiments. For Liu, Fengchao, et al. "Prolonged inhibition of class I PI3K promotes liver cancer stem cell expansion by augmenting SGK3/GSK-3β/β-catenin signalling." Journal of Experimental & Clinical Cancer Research 37.1 (2018): 122. Chen, Rui, et al. "Activation of CD137 Signaling Enhances Vascular Calcification through c-Jun (2018). [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing t lo yp ss e .s ,f o un f c c t e io ll n m :A o c t t il i i n ty s a a n re d h a i r g e h u lybi c q o u n it s o e u r s v l e y d e p xp ro re te s i s n e s d th i a n t a a ll re eu in k v a o ry lv o e t d ic in various cells.,miscellaneous:In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.,similarity:Belongs to the more information, please consult technical personnel. major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008], N-Terminal Kinase-Dependent Disruption of Autophagic Flux." Mediators of inflammation 2018www.dumabio.com Xie, Changli, et al. "Effects of IRF1 and IFN-β interaction on the M1 polarization of macrophages and its antitumor function." International journal of molecular medicine 38.1 (2016): 148-160. Yao, Chong, et al. "Crocin induces autophagic apoptosis in hepatocellular carcinoma by inhibiting Akt/mTOR activity." OncoTargets and therapy 11 (2018): 2017. L In i, tim Ha a i l m H in y g p ,e e rp t l a a l s . ia "C a o n ll d ag Im en pr E o x v t e er R n e a m l S o c d a e f l f i o n l g ds of M V it e ig in ate Grafts in a Rabbit Arteriovenous Graft Model." BioMed Research International 2017 (2017).