GAPDH Polyclonal Antibody

 GAPDH Polyclonal Antibody 

Catalog No DM-Ab-03502

Isotype IgG 

Reactivity Human;Mouse;Rat;Rabbit;Ch;Mk;sheep;X;Fish;Chicken;Guinea pig;Guinea pig;Duck 

Applications WB;IHC;IF 

Gene Name GAPDH 

Protein Name Glyceraldehyde-3-phosphate dehydrogenase 

Immunogen Recombinant Protein of GAPDH 

Specificity The antibody detects endogenous GAPDH protein. 

Formulation PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol. 

Source Polyclonal, Rabbit,IgG 

Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen. 

Dilution WB: 1:5000 IHC: 1:200. IF 1:50-200 

Concentration 1 mg/ml 

Purity ≥90%

Storage Stability -20°C/1 year 

Synonyms GAPDH; GAPD; CDABP0047; OK/SW-cl.12; Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH 

Observed Band 37kD 

Cell Pathway Cytoplasm, cytosol . Nucleus . Cytoplasm, perinuclear region . Membrane . Cytoplasm, cytoskeleton . Translocates to the nucleus following S-nitrosylation 

Tissue Specificity Astrocytoma,Brain,Cajal-Retzius cell,Colon adenocarcinoma,Epitheliu and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions (PubMed:12829261). .

Function catalytic activity:D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = glyceraldehyde-3-phosphate dehydrogenase fami 

Background glyceraldehyde-3-phosphate dehydrogenase(GAPDH) Homo sapiens This

matters needing attention Avoid repeated freezing and thawing! 

Usage suggestions This product can be used in immunological reaction related experiments. For more information, please consult technical personnel. Zhao, Shaorong et al. “Deciphering the performance of polo-like kinase 1 in triple-negative breast cancer progression according to the centromere protein U-phosphorylation pathway.” American journal of cancer research vol. 11,5 2142-2158. 15 May. 2021 3-phospho-D-glyceroyl phosphate + NADH.,function:Independent of its glycolytic activity it is also involved in membrane trafficking in the early secretory pathway.,online information:Glyceraldehyde 3-phosphate dehydrogenase entry,pathway:Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1.,pathway:Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.,PTM:Reversible S-nitrosylation of Cys-152 inhibits enzymatic activity and increases endogenous ADP-ribosylation, which inhibits the enzyme in a non-reversible manner. The latter modification is more likely to be a pathophysiological event associated with inhibition of gluconeogenesis.,sequence caution:Differs quite extensively.,similarity:Belongs to the gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferriZhao, TingTing, et al. "Tangshen formula modulates gut Microbiota and reduces gut-derived toxins in diabetic nephropathy rats." Biomedicine & Pharmacotherapy 129 (2020): 110325. Yi, Sheng, et al. "Peripheral nerve injury induces dynamic changes of tight junction components." Frontiers in physiology9 (2018): 1519. Chang, Ying, et al. "Lentivirus-mediated knockdown of astrocyte elevated gene-1 inhibits growth and induces a re p t o in p o to b s la is st t o h m ro a ug c h el M ls.A "P P K loS pa o th n w e a 1 y1 s .2 in (2 h 0 u 1 m 6 a ): n e0148763. Wang, Xiao, et al. "Knockdown of Phospholipase Cɛ (PLCɛ) Inhibits Cell Proliferation via Phosphatase and Tensin Homolog Deleted on Chromosome 10 (PTEN)/AKT Signaling Pathway in Human Prostate Cancer." Medical science monitor: international medical journal of experimental and clinical research 24 (2018): 254.