ZN143 rabbit pAb

CTCF Monoclonal Antibody 

Catalog No  DM-00964 

Isotype IgG 

Reactivity Human;Monkey 

Applications WB;ELISA 

Gene Name CTCF 

Protein Name Transcriptional repressor CTCF

Immunogen Purified recombinant fragment of human CTCF expressed in E. Coli. 

Specificity CTCF Monoclonal Antibody detects endogenous levels of CTCF protein. 

Formulation Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol. 

Source Monoclonal, Mouse 

Purification Affinity purification

Dilution Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. 

Concentration 1 mg/ml 

Purity ≥90% 

Storage Stability -20°C/1 year 

Synonyms CTCF; Transcriptional repressor CTCF; 11-zinc finger protein; CCCTC-binding factor; CTCFL paralog 

Observed Band Cell Pathway Nucleus, nucleoplasm . Chromosome . Chromosome, centromere . May translocate to the nucleolus upon cell differentiation. Associates with both centromeres and chromosomal arms during metaphase. Associates with the H19 ICR in mitotic chromosomes. May be preferentially excluded from heterochromatin during interphase. 

Tissue Specificity Ubiquitous. Absent in primary spermatocytes. 

Function function:Transcriptional repressor binding to promoters of vertebrate c-myc gene. Also binds to the PLK and PIM1 promoters. May prevent the access of transcriptional activators to enhancers. Also acts as a transcriptional activator of APP. Involved in different aspects of gene regulation including promoter activation or repression, hormone-responsive gene silencing, methylation-dependent chromatin insulation, and genomic imprinting. Seems to act as tumor suppressor.,similarity:Belongs to the CTCF zinc-finger protein family.,similarity:Contains 11 C2H2-type zinc fingers.,subunit:Interacts with CHD8.,tissue specificity:Ubiquitous. Absent in primary spermatocytes.,

Background This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combination