FITC标记的广谱细胞角蛋白PCK抗体

Host: Rabbit                                            Isotype: IgG

Clonality: Polyclonal

Target: pan CK | pan Cytokeratin

Purification: affinity purified by Protein A Concentration: 1mg/ml

Storage: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background: Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic

intermediate filament proteins, including K9–K23, and the hair

keratins Ha1–Ha8. Type II keratins are the basic or neutral

courterparts to the acidic type I keratins, including K1–K8, and the hair keratins, Hb1–Hb6. In the cytoplasm, the keratin filaments

conform a complex network which extends from the surface of the nucleus to the cell membrane. Apart from the relatively static

functions provided in terms of supporting the nucleus and

providing tensile strength to the cell, the cytokeratin networks

undergo rapid phosphate exchanges mediated depolymerization, with important implications in the more dynamic cellular

processes such as mitosis and post-mitotic period, cell movement and differentiation.

Cytokeratin pan is part of a subfamily of intermediate filament

proteins that are characterized by remarkable biochemical

diversity, and represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight

between 40 kDa- 68 kDa, and an isoelectric pH between 4.9-7.8.

The individual human cytokeratins are numbered 1 to 20. The

various epithelia in the human body usually express cytokeratins  which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an

epithelium. Cytokeratin subtype expression patterns are used to

an increasing extent in the distinction of different types of

epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using

immunohistochemistry on tissue sections, but are also a useful

tool in cytopathology and flow cytometric assays. The composition of cytokeratin pairs vary with the epithelial cell type, stage of

differentiation, cellular growth environment, and disease state.

Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Applications: IF (1:100-500)

Flow-Cyt (1μg /test)

ICC/IF (1:100-500) Excitation Spectrum: 495nm

Emission spectrum: 519nm

Reactivity: Human, Mouse, Rat

(predicted: Rabbit, Pig,Cow, Chicken, Dog,Horse)